Long-read RGASP

LRGASP logoThe Long-read RNA-seq Genome Annotation Assessment Project (LRGASP) Consortium is organizing a systematic evaluation of different methods for transcript computational identification and quantification using long-read sequencing technologies such as PacBio and Oxford Nanopore. We are interested in characterizing the strengths and potential remaining challenges in using these technologies to annotate and quantify the transcriptomes of both model and non-model organisms.

The consortium will generate cDNA and direct RNA datasets using different platforms and protocols in human, mouse, and manatee samples. Participants will be provided with the data to generate annotations of expressed genes and transcripts as well as measure their expression levels. Evaluators from different institutions will determine which pipelines have the highest accuracy for different aspects that include transcription detection, quantification and differential expression.

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LRGASP challenge now open

The Long-read RNA-seq Genome Annotation Assessment Project challenge has now launched.

Data is now available here:


> Registration on Synapse for Submissions

> LRGASP Guidelines for Submission
 Presentation on LRGASP submissions

The deadline for submissions has been extended to 8 October 2021.

Join this community effort and submit your predictions of transcript models and transcript quantification.

> List of consortium organizers

Detailed information (updated):

> Description of LRGASP Data and Evaluation

LRGASP evaluation results

> Challenge 1 preliminary results

> Performance evaluation in challenge 2

> Challenge 3 preliminary results

Recordings of consortium discussions

> September 2020

> February 2021

> May 2021

> September 2021

> February 2022 (Challenge 1)

> March 2022 (Challenge 2)

> March 2022 (Challenge 3)