The Long-read RNA-seq Genome Annotation Assessment Project (LRGASP) Consortium is organizing a systematic evaluation of different methods for transcript computational identification and quantification using long-read sequencing technologies such as PacBio and Oxford Nanopore. We are interested in characterizing the strengths and potential remaining challenges in using these technologies to annotate and quantify the transcriptomes of both model and non-model organisms.
The consortium will generate cDNA and direct RNA datasets using different platforms and protocols in human, mouse, and manatee samples. Participants will be provided with the data to generate annotations of expressed genes and transcripts as well as measure their expression levels. Evaluators from different institutions will determine which pipelines have the highest accuracy for different aspects that include transcription detection, quantification and differential expression.
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LRGASP challenge now open
The Long-read RNA-seq Genome Annotation Assessment Project challenge has now launched.
Data is now available here:
The deadline for submissions has been extended to 8 October 2021.
Join this community effort and submit your predictions of transcript models and transcript quantification.
Detailed information (updated):
LRGASP evaluation results
Recordings of consortium discussions
> May 2021